| AatII | 5 | 1 | GACGTC |
| AbaSI | 12 | 10 | Cnnnnnnnnnnn |
| AccI | 2 | 4 | GTmkAC |
| Acc65I | 1 | 5 | GGTACC |
| AciI | 1 | 3 | CCGC |
| AclI | 2 | 4 | AACGTT |
| AcuI | 22 | 20 | CTGAAGnnnnnnnnnnnnnnnn |
| AfeI | 3 | 3 | AGCGCT |
| AflII | 1 | 5 | CTTAAG |
| AflIII | 1 | 5 | ACryGT |
| AgeI | 1 | 5 | ACCGGT |
| AhdI | 6 | 5 | GACnnnnnGTC |
| AleI | 5 | 5 | CACnnnnGTG |
| AluI | 2 | 2 | AGCT |
| AlwI | 9 | 10 | GGATCnnnnn |
| AlwNI | 6 | 3 | CAGnnnCTG |
| ApaI | 5 | 1 | GGGCCC |
| ApaLI | 1 | 5 | GTGCAC |
| ApeKI | 1 | 4 | GCwGC |
| ApoI | 1 | 5 | rAATTy |
| AscI | 2 | 6 | GGCGCGCC |
| AseI | 2 | 4 | ATTAAT |
| AsiSI | 5 | 3 | GCGATCGC |
| AvaI | 1 | 5 | CyCGrG |
| AvaII | 1 | 4 | GGwCC |
| AvrII | 1 | 5 | CCTAGG |
| BaeI | 23 | 18 | ACnnnnGTAyCnnnnnnnnnnnn |
| BaeI | 26 | 21 | GrTACnnnnGTnnnnnnnnnnnnnnn |
| BaeGI | 5 | 1 | GkGCmC |
| BamHI | 1 | 5 | GGATCC |
| BanI | 1 | 5 | GGyrCC |
| BanII | 5 | 1 | GrGCyC |
| BbsI | 8 | 12 | GAAGACnnnnnn |
| BbvI | 13 | 17 | GCAGCnnnnnnnnnnnn |
| BbvCI | 2 | 5 | CCTCAGC |
| BccI | 9 | 10 | CCATCnnnnn |
| BceAI | 17 | 19 | ACGGCnnnnnnnnnnnnnn |
| BcgI | 24 | 22 | CGAnnnnnnTGCnnnnnnnnnnnn |
| BcgI | 24 | 22 | GCAnnnnnnTCGnnnnnnnnnnnn |
| BciVI | 12 | 11 | GTATCCnnnnnn |
| BclI | 1 | 5 | TGATCA |
| BcoDI | 6 | 10 | GTCTCnnnnn |
| BfaI | 1 | 3 | CTAG |
| BfuAI | 10 | 14 | ACCTGCnnnnnnnn |
| BglI | 7 | 4 | GCCnnnnnGGC |
| BglII | 1 | 5 | AGATCT |
| BlpI | 2 | 5 | GCTnAGC |
| BmgBI | 3 | 3 | CACGTC |
| BmrI | 11 | 10 | ACTGGGnnnnn |
| BmtI | 5 | 1 | GCTAGC |
| BpmI | 22 | 20 | CTGGAGnnnnnnnnnnnnnnnn |
| Bpu10I | 2 | 5 | CCTnAGC |
| BpuEI | 22 | 20 | CTTGAGnnnnnnnnnnnnnnnn |
| BsaI | 7 | 11 | GGTCTCnnnnn |
| BsaAI | 3 | 3 | yACGTr |
| BsaBI | 5 | 5 | GATnnnnATC |
| BsaHI | 2 | 4 | GrCGyC |
| BsaJI | 1 | 5 | CCnnGG |
| BsaWI | 1 | 5 | wCCGGw |
| BsaXI | 21 | 18 | ACnnnnnCTCCnnnnnnnnnn |
| BsaXI | 23 | 20 | GGAGnnnnnGTnnnnnnnnnnnn |
| BseRI | 16 | 14 | GAGGAGnnnnnnnnnn |
| BseYI | 1 | 5 | CCCAGC |
| BsgI | 22 | 20 | GTGCAGnnnnnnnnnnnnnnnn |
| BsiEI | 4 | 2 | CGryCG |
| BsiHKAI | 5 | 1 | GwGCwC |
| BsiWI | 1 | 5 | CGTACG |
| BslI | 7 | 4 | CCnnnnnnnGG |
| BsmI | 7 | 5 | GAATGCn |
| BsmAI | 6 | 10 | GTCTCnnnnn |
| BsmBI | 7 | 11 | CGTCTCnnnnn |
| BsmFI | 15 | 19 | GGGACnnnnnnnnnnnnnn |
| BsoBI | 1 | 5 | CyCGrG |
| Bsp1286I | 5 | 1 | GdGChC |
| BspCNI | 14 | 12 | CTCAGnnnnnnnnn |
| BspDI | 2 | 4 | ATCGAT |
| BspEI | 1 | 5 | TCCGGA |
| BspHI | 1 | 5 | TCATGA |
| BspMI | 10 | 14 | ACCTGCnnnnnnnn |
| BspQI | 8 | 11 | GCTCTTCnnnn |
| BsrI | 6 | 4 | ACTGGn |
| BsrBI | 3 | 3 | CCGCTC |
| BsrDI | 8 | 6 | GCAATGnn |
| BsrFI | 1 | 5 | rCCGGy |
| BsrGI | 1 | 5 | TGTACA |
| BssHII | 1 | 5 | GCGCGC |
| BssSI | 1 | 5 | CACGAG |
| BstAPI | 7 | 4 | GCAnnnnnTGC |
| BstBI | 2 | 4 | TTCGAA |
| BstEII | 1 | 6 | GGTnACC |
| BstNI | 2 | 3 | CCwGG |
| BstUI | 2 | 2 | CGCG |
| BstXI | 8 | 4 | CCAnnnnnnTGG |
| BstYI | 1 | 5 | rGATCy |
| BstZ17I | 3 | 3 | GTATAC |
| Bsu36I | 2 | 5 | CCTnAGG |
| BtgI | 1 | 5 | CCryGG |
| BtgZI | 16 | 20 | GCGATGnnnnnnnnnnnnnn |
| BtsI | 8 | 6 | GCAGTGnn |
| BtsIMutI | 7 | 5 | CAGTGnn |
| BtsCI | 7 | 5 | GGATGnn |
| Cac8I | 3 | 3 | GCnnGC |
| ClaI | 2 | 4 | ATCGAT |
| CspCI | 24 | 22 | CAAnnnnnGTGGnnnnnnnnnnnn |
| CspCI | 25 | 23 | CCACnnnnnTTGnnnnnnnnnnnnn |
| CviAII | 1 | 3 | CATG |
| CviKI-1 | 2 | 2 | rGCy |
| CviQI | 1 | 3 | GTAC |
| DdeI | 1 | 4 | CTnAG |
| DpnI | 2 | 2 | GATC |
| DpnII | 0 | 4 | GATC |
| DraI | 3 | 3 | TTTAAA |
| DraIII | 6 | 3 | CACnnnGTG |
| DrdI | 7 | 5 | GACnnnnnnGTC |
| EaeI | 1 | 5 | yGGCCr |
| EagI | 1 | 5 | CGGCCG |
| EarI | 7 | 10 | CTCTTCnnnn |
| EciI | 17 | 15 | GGCGGAnnnnnnnnnnn |
| EcoNI | 5 | 6 | CCTnnnnnAGG |
| EcoO109I | 2 | 5 | rGGnCCy |
| EcoRI | 1 | 5 | GAATTC |
| EcoRV | 3 | 3 | GATATC |
| Eco53kI | 3 | 3 | GAGCTC |
| Esp3I | 7 | 11 | CGTCTCnnnnn |
| FatI | 0 | 4 | CATG |
| FauI | 9 | 11 | CCCGCnnnnnn |
| Fnu4HI | 2 | 3 | GCnGC |
| FokI | 14 | 18 | GGATGnnnnnnnnnnnnn |
| FseI | 6 | 2 | GGCCGGCC |
| FspI | 3 | 3 | TGCGCA |
| FspEI | 14 | 18 | CCnnnnnnnnnnnnnnnn |
| HaeII | 5 | 1 | rGCGCy |
| HaeIII | 2 | 2 | GGCC |
| HgaI | 10 | 15 | GACGCnnnnnnnnnn |
| HhaI | 3 | 1 | GCGC |
| HinP1I | 1 | 3 | GCGC |
| HincII | 3 | 3 | GTyrAC |
| HindIII | 1 | 5 | AAGCTT |
| HinfI | 1 | 4 | GAnTC |
| HpaI | 3 | 3 | GTTAAC |
| HpaII | 1 | 3 | CCGG |
| HphI | 13 | 12 | GGTGAnnnnnnnn |
| Hpy99I | 5 | 0 | CGwCG |
| Hpy166II | 3 | 3 | GTnnAC |
| Hpy188I | 3 | 2 | TCnGA |
| Hpy188III | 2 | 4 | TCnnGA |
| HpyAV | 11 | 10 | CCTTCnnnnnn |
| HpyCH4III | 3 | 2 | ACnGT |
| HpyCH4IV | 1 | 3 | ACGT |
| HpyCH4V | 2 | 2 | TGCA |
| KasI | 1 | 5 | GGCGCC |
| KpnI | 5 | 1 | GGTACC |
| LpnPI | 14 | 18 | CCdGnnnnnnnnnnnnnn |
| MboI | 0 | 4 | GATC |
| MboII | 13 | 12 | GAAGAnnnnnnnn |
| MfeI | 1 | 5 | CAATTG |
| MluI | 1 | 5 | ACGCGT |
| MluCI | 0 | 4 | AATT |
| MlyI | 10 | 10 | GAGTCnnnnn |
| MmeI | 26 | 24 | TCCrACnnnnnnnnnnnnnnnnnnnn |
| MnlI | 11 | 10 | CCTCnnnnnnn |
| MscI | 3 | 3 | TGGCCA |
| MseI | 1 | 3 | TTAA |
| MslI | 5 | 5 | CAynnnnrTG |
| MspI | 1 | 3 | CCGG |
| MspA1I | 3 | 3 | CmGCkG |
| MspJI | 13 | 17 | Cnnrnnnnnnnnnnnnn |
| MwoI | 7 | 4 | GCnnnnnnnGC |
| NaeI | 3 | 3 | GCCGGC |
| NarI | 2 | 4 | GGCGCC |
| NciI | 2 | 3 | CCsGG |
| NcoI | 1 | 5 | CCATGG |
| NdeI | 2 | 4 | CATATG |
| NgoMIV | 1 | 5 | GCCGGC |
| NheI | 1 | 5 | GCTAGC |
| NlaIII | 4 | 0 | CATG |
| NlaIV | 3 | 3 | GGnnCC |
| NmeAIII | 27 | 25 | GCCGAGnnnnnnnnnnnnnnnnnnnnn |
| NotI | 2 | 6 | GCGGCCGC |
| NruI | 3 | 3 | TCGCGA |
| NsiI | 5 | 1 | ATGCAT |
| NspI | 5 | 1 | rCATGy |
| PacI | 5 | 3 | TTAATTAA |
| PaeR7I | 1 | 5 | CTCGAG |
| PaqCI | 11 | 15 | CACCTGCnnnnnnnn |
| PciI | 1 | 5 | ACATGT |
| PflFI | 4 | 5 | GACnnnGTC |
| PflMI | 7 | 4 | CCAnnnnnTGG |
| PleI | 9 | 10 | GAGTCnnnnn |
| PluTI | 5 | 1 | GGCGCC |
| PmeI | 4 | 4 | GTTTAAAC |
| PmlI | 3 | 3 | CACGTG |
| PpuMI | 2 | 5 | rGGwCCy |
| PshAI | 5 | 5 | GACnnnnGTC |
| PsiI | 3 | 3 | TTATAA |
| PspGI | 0 | 5 | CCwGG |
| PspOMI | 1 | 5 | GGGCCC |
| PspXI | 2 | 6 | vCTCGAGb |
| PstI | 5 | 1 | CTGCAG |
| PvuI | 4 | 2 | CGATCG |
| PvuII | 3 | 3 | CAGCTG |
| RsaI | 2 | 2 | GTAC |
| RsrII | 2 | 5 | CGGwCCG |
| SacI | 5 | 1 | GAGCTC |
| SacII | 4 | 2 | CCGCGG |
| SalI | 1 | 5 | GTCGAC |
| SapI | 8 | 11 | GCTCTTCnnnn |
| Sau96I | 1 | 4 | GGnCC |
| Sau3AI | 0 | 4 | GATC |
| SbfI | 6 | 2 | CCTGCAGG |
| ScaI | 3 | 3 | AGTACT |
| ScrFI | 2 | 3 | CCnGG |
| SexAI | 1 | 6 | ACCwGGT |
| SfaNI | 10 | 14 | GCATCnnnnnnnnn |
| SfcI | 1 | 5 | CTryAG |
| SfiI | 8 | 5 | GGCCnnnnnGGCC |
| SfoI | 3 | 3 | GGCGCC |
| SgrAI | 2 | 6 | CrCCGGyG |
| SmaI | 3 | 3 | CCCGGG |
| SmlI | 1 | 5 | CTyrAG |
| SnaBI | 3 | 3 | TACGTA |
| SpeI | 1 | 5 | ACTAGT |
| SphI | 5 | 1 | GCATGC |
| SrfI | 4 | 4 | GCCCGGGC |
| SspI | 3 | 3 | AATATT |
| StuI | 3 | 3 | AGGCCT |
| StyI | 1 | 5 | CCwwGG |
| StyD4I | 0 | 5 | CCnGG |
| SwaI | 4 | 4 | ATTTAAAT |
| TaqI | 1 | 3 | TCGA |
| TfiI | 1 | 4 | GAwTC |
| TseI | 1 | 4 | GCwGC |
| Tsp45I | 0 | 5 | GTsAC |
| TspMI | 1 | 5 | CCCGGG |
| TspRI | 9 | 0 | nnCAsTGnn |
| Tth111I | 4 | 5 | GACnnnGTC |
| XbaI | 1 | 5 | TCTAGA |
| XcmI | 8 | 7 | CCAnnnnnnnnnTGG |
| XhoI | 1 | 5 | CTCGAG |
| XmaI | 1 | 5 | CCCGGG |
| XmnI | 5 | 5 | GAAnnnnTTC |
| ZraI | 3 | 3 | GACGTC |